□ Information for submitting proposals

The protein crystal screening (PCS) beamtime at the PAL-XFEL is open for domestic scientists who are willing to judge a crystal quality prior to the macromolecular crystallography experiments at not only XFEL but synchrotron facilities. To maximize operational efficiency, the PCS beamtime will be operated with standard fixed target sample delivery instruments at specific X-ray energy. The allocated beamtime, including sample preparation and loading, is about an hour for each accepted proposal. In particular, proposals planning to apply for the PAL-XFEL regular beamtime will get a higher priority.
Please register as a user of PAL and submit an application form for the PAL-XFEL proposal via the website (http://pal.postech.ac.kr/).

 

□ Guidelines for preparing PCS beamtime proposal

○ Experiment schedule

- Proposal deadline: September 26, 2022 (Korean Standard Time)

- Experiments: January – June 2023

○ Description of the instrumentations for crystal screening

	Descriptions for PCS
SFX @NCI	⦁ XFEL energy: 9.5 keV (fixed) ⦁ K-B focused XFEL beamsize: H × V ~ 5 × 5 μm2 ⦁ High resolution optical microscope Hirox KH-8700 + MXG-2500 lens (special order) Magnification: 35x ~ 5000x, FOV: 0.04 mm @ 5000x ⦁ Sample chamber and delivery: fixed target system** ⦁ Rayonix MX225-HS or Jungfrau 4M detector ⦁ Data acquisition & analysis software: OnDA, Cheetah, cctbx, CrystFEL, Phenix, CCP4i

**D. Lee et al., Scientific Reports 9, 6971 (2019)

 

○ Contact information

Users must contact Dr. Jaehyun Park (jaehyun.park@postech.ac.kr) or Dr. Sang Jae Lee (sangjaelee@postech.ac.kr) to discuss the details of technical issues and specifics related to the experiment.

PAL-XFEL PCS (Protein Crystal Screening) proposal

(Less than 2 pages)

 

1. Title

 

2. Research team

• Name, affiliation, and position with the email address

(All members are required to do PAL registration and get radiation safety training)

• Brief description on roles of members (experiment, sample handling, etc.)

 

3. Scientific importance

• Scientific uniqueness of the proposed research

• Expected scientific or industrial impact

 

4. Experimental procedure and samples

 

5. Description about whether to apply for the PAL-XFEL regular beamtime

 

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* The PCS application form is allowed in both Korean and English.

* The preliminary diffraction data collected at the PAL-XFEL or synchrotron are

recommended to be submitted as a supplementary information for regular SFX beamtime

application.

* Fee: 20,000 KRW / 1 hr (Non-proprietary research)

2023 1st PAL-XFEL Call for PCS Proposal.pdf (83.74KB)